Posted by
ctrueden on
Apr 06, 2015; 3:42pm
URL: http://imagej.273.s1.nabble.com/LSM-Split-Channels-Broken-tp5012281p5012369.html
Hi Jacob, Ignacio & everyone,
> Two things have recently happened with my LSM files:
>
> -I now need to import rather than just opening them
>
> -Split channels does not work
I cannot reproduce on my system:
* Download dataset from
http://loci.wisc.edu/sample-data/2chzt* Unzip it
* Launch Fiji, File > Open, choose 2chZT.lsm
If 2chZT.lsm works for you, but you have data which does not, please upload
a non-working file using the Help > Upload Sample Image command, then reply
back with the name of the file you uploaded.
Regards,
Curtis
On Thu, Apr 2, 2015 at 3:20 AM, Ignacio Arganda-Carreras <
[hidden email]> wrote:
> Hello Rebecca,
>
> I observed the same problems.
>
> The channel splitting can be fixed if you convert the image first to a
> Composite (in a macro run("Make Composite", "display=Composite"); ). But I
> agree it shouldn't be like this.
>
> ignacio
>
> On Thu, Apr 2, 2015 at 1:35 AM, Rebecca Keller <
[hidden email]>
> wrote:
>
> > A follow-up to this problem: for some reason, FIJI thinks my images are
> > interleaved rather than separate channels, even though they appear with a
> > channel-slider--I've been able to separate the channels by using
> > "deinterleave" rather than "split channels." I think this is probably a
> > bona fide bug...
> >
> > JPK
> >
> > On Tue, Mar 31, 2015 at 9:51 PM, Rebecca Keller <
[hidden email]>
> > wrote:
> >
> > > Dear Imagers,
> > >
> > > Two things have recently happened with my LSM files:
> > >
> > > -I now need to import rather than just opening them
> > >
> > > -Split channels does not work; complains with "multichannel image
> > > required." (But my images are multichannel, and I can see both with the
> > > slider.)
> > >
> > > Neither of these was a problem before--any thoughts as to what's going
> > on?
> > >
> > > JPK
> > >
> >
> > --
> > ImageJ mailing list:
http://imagej.nih.gov/ij/list.html> >
>
>
>
> --
> Ignacio Arganda-Carreras, Ph.D.
> Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech
> Bâtiment 2
> INRA Centre de Versailles-Grignon
> Route de St-Cyr (RD10)
> 78026 Versailles Cedex France
>
> Tel : +33 (0)1 30 83 30 00 - fax : +33 (0)1 30 83 33 19
> Website:
http://sites.google.com/site/iargandacarreras/> <
http://biocomp.cnb.csic.es/~iarganda/index_EN.html>
>
> --
> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html>
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