Choose a region automatically and quantify black pixels

Posted by ram prasad on
URL: http://imagej.273.s1.nabble.com/Choose-a-region-automatically-and-quantify-black-pixels-tp5012430.html

Hey All,

I'm trying to quantify black colour on the fore wings of butterflies. I would
like to do this by calculating the ratio of the area occupied by the black
pixels/total number of pixels in the wing. I would really appreciate it if
you could tell me how I should go about it. Is there a way to tell ImageJ
to find the edges of all objects in an image and pick an object with a
certain shape?

Also, since I have hundred of images I would like to employ batch mode
to analyze
them and I would like to know if that is possible. Basically, I want the
software to detect the fore wings by itself I'm not sure if this is
feasible.

I have attached an image for your reference. Any help will be really
appreciated.

Thanks,
Ram

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