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Red-yellow-green measurement on fluorescent images

Posted by Simon-Stott on Apr 10, 2015; 10:08am
URL: http://imagej.273.s1.nabble.com/Red-yellow-green-measurement-on-fluorescent-images-tp5012431.html

Hi,
I would like to know if it is possible to set up a colour spectrum measurement for an image so that red=1 yellow=0 and green=-1, thus allowing us to use the point tool in imagej/fiji to measure the level of co-expression between cell somas on a fluorescent image such as the one attached.


(Image is Tyrosine hydroxylase (dopamine neurons) in the red channel and calbindin (calcium binding protein) in the green channel on a coronal section of primate midbrain.

We are trying to identify subsets of midbrain dopamine neurons, and would like to offer a semi-quantitative measure indicating levels of expression in each subpopulation of cells. Any help would be greatly appreciated.
Simon