Re: Red-yellow-green measurement on fluorescent images

Posted by Simon-Stott on Apr 11, 2015; 7:43pm
URL: http://imagej.273.s1.nabble.com/Red-yellow-green-measurement-on-fluorescent-images-tp5012431p5012440.html

I should add that our images are not confocal. The sections of primate midbrain are huge and we have approximately 20 of them across the A-P axis of the midbrain (series of 10; 35um thick). To cover all of the area of the dopamine neurons we are using the automated Olympus bx61 platform (x20 len with 0.35 micron per pixel resolution). Confocal microscopy together with JaCoP would take literally forever to do across all of the sections. The automated scanning approach has sped things up, but determining a semi-quantitative measure of colocalization is proving difficult, especially with some of the calcium binding proteins which have varying levels of expression between cells.