Re: LSM Split Channels Broken

Posted by Ignacio Arganda-Carreras-2 on Apr 13, 2015; 9:04am
URL: http://imagej.273.s1.nabble.com/LSM-Split-Channels-Broken-tp5012281p5012450.html

Sorry for the late answer. I think this problem got fixed with the updates
of bioformats during the last week.

Cheers!

ignacio

On Mon, Apr 6, 2015 at 5:42 PM, Curtis Rueden <[hidden email]> wrote:

> Hi Jacob, Ignacio & everyone,
>
> > Two things have recently happened with my LSM files:
> >
> > -I now need to import rather than just opening them
> >
> > -Split channels does not work
>
> I cannot reproduce on my system:
>
> * Download dataset from http://loci.wisc.edu/sample-data/2chzt
> * Unzip it
> * Launch Fiji, File > Open, choose 2chZT.lsm
>
> If 2chZT.lsm works for you, but you have data which does not, please upload
> a non-working file using the Help > Upload Sample Image command, then reply
> back with the name of the file you uploaded.
>
> Regards,
> Curtis
>
> On Thu, Apr 2, 2015 at 3:20 AM, Ignacio Arganda-Carreras <
> [hidden email]> wrote:
>
> > Hello Rebecca,
> >
> > I observed the same problems.
> >
> > The channel splitting can be fixed if you convert the image first to a
> > Composite (in a macro run("Make Composite", "display=Composite"); ). But
> I
> > agree it shouldn't be like this.
> >
> > ignacio
> >
> > On Thu, Apr 2, 2015 at 1:35 AM, Rebecca Keller <[hidden email]>
> > wrote:
> >
> > > A follow-up to this problem: for some reason, FIJI thinks my images are
> > > interleaved rather than separate channels, even though they appear
> with a
> > > channel-slider--I've been able to separate the channels by using
> > > "deinterleave" rather than "split channels." I think this is probably a
> > > bona fide bug...
> > >
> > > JPK
> > >
> > > On Tue, Mar 31, 2015 at 9:51 PM, Rebecca Keller <[hidden email]>
> > > wrote:
> > >
> > > > Dear Imagers,
> > > >
> > > > Two things have recently happened with my LSM files:
> > > >
> > > > -I now need to import rather than just opening them
> > > >
> > > > -Split channels does not work; complains with "multichannel image
> > > > required." (But my images are multichannel, and I can see both with
> the
> > > > slider.)
> > > >
> > > > Neither of these was a problem before--any thoughts as to what's
> going
> > > on?
> > > >
> > > > JPK
> > > >
> > >
> > > --
> > > ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> > >
> >
> >
> >
> > --
> > Ignacio Arganda-Carreras, Ph.D.
> > Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech
> > Bâtiment 2
> > INRA Centre de Versailles-Grignon
> > Route de St-Cyr (RD10)
> > 78026 Versailles Cedex France
> >
> > Tel : [phone] - fax : [phone]
> > Website: http://sites.google.com/site/iargandacarreras/
> > <http://biocomp.cnb.csic.es/~iarganda/index_EN.html>
> >
> > --
> > ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> >
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--
Ignacio Arganda-Carreras, Ph.D.
Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech
Bâtiment 2
INRA Centre de Versailles-Grignon
Route de St-Cyr (RD10)
78026 Versailles Cedex France

Tel : [phone] - fax : [phone]
Website: http://sites.google.com/site/iargandacarreras/
<http://biocomp.cnb.csic.es/~iarganda/index_EN.html>

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