Re: Simple Neurite Tracer plugin - analyze skeleton

Posted by Jan Eglinger on
URL: http://imagej.273.s1.nabble.com/Simple-Neurite-Tracer-plugin-analyze-skeleton-tp5012441p5012455.html

Hi Ignacio and Rotem,

this bug can be fixed by keeping the calibration data of the original
image when creating the path image. I filed a pull request here:
https://github.com/fiji/Simple_Neurite_Tracer/pull/2

Jan


On 13.04.2015 10:26, Ignacio Arganda-Carreras wrote:

> Hello Rotem,
>
> That's indeed a bug. It should be fixed within the code but for now, what
> you can do is to call Analayze Skeleton with the option to display the
> skeletons, change the calibration of the skeletons manually (Image >
> Properties) and then call the Analyze Skeleton plugin from the main menu.
>
> I hope this helps!
>
> ignacio
>
> On Sun, Apr 12, 2015 at 11:04 AM, Rotem Oved <
> [hidden email]> wrote:
>
>>    Hi all,
>> I'm trying to use the plugin called"simple neurite tracer" on fiji (under
>> "segmentation"), tomeasure filaments length mostly. The plugin does seem to
>> use the informationstored at the images in order to set a scale, and at the
>> beginning, at thewindow called "all paths", it shows me the measurements in
>> microns,just as I want them. However, when I go at the plugin's window to
>> analysis-> analyze skeleton, the information that is given then does not
>> match theprevious information, and we believe it is given in pixels (I
>> attached an imageto demonstrate). Is there any way to convert that, so that
>> all the informationwill appear in microns?
>> If something is unclear I would be morethan happy to explain what I meant.
>> Thank you very much.
>>

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