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Re: Simple Neurite Tracer plugin - analyze skeleton

Posted by Ignacio Arganda-Carreras-2 on Apr 13, 2015; 1:23pm
URL: http://imagej.273.s1.nabble.com/Simple-Neurite-Tracer-plugin-analyze-skeleton-tp5012441p5012456.html

Thanks, Jan, you were faster than me ;)

On Mon, Apr 13, 2015 at 3:19 PM, Jan Eglinger <[hidden email]>
wrote:

> Hi Ignacio and Rotem,
>
> this bug can be fixed by keeping the calibration data of the original
> image when creating the path image. I filed a pull request here:
> https://github.com/fiji/Simple_Neurite_Tracer/pull/2
>
> Jan
>
>
>
> On 13.04.2015 10:26, Ignacio Arganda-Carreras wrote:
>
>> Hello Rotem,
>>
>> That's indeed a bug. It should be fixed within the code but for now, what
>> you can do is to call Analayze Skeleton with the option to display the
>> skeletons, change the calibration of the skeletons manually (Image >
>> Properties) and then call the Analyze Skeleton plugin from the main menu.
>>
>> I hope this helps!
>>
>> ignacio
>>
>> On Sun, Apr 12, 2015 at 11:04 AM, Rotem Oved <
>> [hidden email]> wrote:
>>
>>     Hi all,
>>> I'm trying to use the plugin called"simple neurite tracer" on fiji (under
>>> "segmentation"), tomeasure filaments length mostly. The plugin does seem
>>> to
>>> use the informationstored at the images in order to set a scale, and at
>>> the
>>> beginning, at thewindow called "all paths", it shows me the measurements
>>> in
>>> microns,just as I want them. However, when I go at the plugin's window to
>>> analysis-> analyze skeleton, the information that is given then does not
>>> match theprevious information, and we believe it is given in pixels (I
>>> attached an imageto demonstrate). Is there any way to convert that, so
>>> that
>>> all the informationwill appear in microns?
>>> If something is unclear I would be morethan happy to explain what I
>>> meant.
>>> Thank you very much.
>>>
>>>


--
Ignacio Arganda-Carreras, Ph.D.
Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech
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78026 Versailles Cedex France

Tel : +33 (0)1 30 83 30 00 - fax : +33 (0)1 30 83 33 19
Website: http://sites.google.com/site/iargandacarreras/
<http://biocomp.cnb.csic.es/~iarganda/index_EN.html>

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