Posted by
Lachlan Whitehead on
Apr 27, 2015; 4:03am
URL: http://imagej.273.s1.nabble.com/Analyze-particles-bug-tp5012617.html
Hi All,
After running my morning update of FIJI I seem to be experiencing a bug in the Analyze Particles function which seems to provide a list of many more particles than it should.
What it appears to be doing is detecting objects correctly, but then assigning every pixel within each object as a separate object (with an area of 0 interestingly).
Needless to say this is causing all sorts of havoc in my analysis routines.
To replicate the bug you can do the following:
- Set Measurements to include "Limit to Threshold"*
- Open blobs test image (or any other image)
- Threshold and analyze particles (display results and/or add to ROI manager to see what I'm talking about).
I've tried this on three PCs running win7, and have had a colleague check it out too.
Any insights would be greatly appreciated.
Thanks,
-Lachie
* Unsetting "limit to threshold" was found to be a workaround, but will be particularly annoying if I have to alter all my previous macros to unset this setting only to re-set it when performing other measurement steps
--
Lachlan Whitehead PhD
BioImage Analyst
Centre for Dynamic Imaging
The Walter & Eliza Hall Institute
1G Royal Parade, Parkville
Victoria 3052, Australia
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