Gel Plots

Posted by Mulcahy, Daniel on Apr 28, 2015; 2:46pm
URL: http://imagej.273.s1.nabble.com/Gel-Plots-tp5012640.html

Hi all,

I am trying to quantify the areas above and below (to the left and right) of DNA images on a gel, on either side of a 9kb fragment on a ladder.  Does anyone know if there is a way to scale all peaks up a little bit, such that the horizontal line never touches the x-axis?  Otherwise, this makes calculating that area very tedious if the line touches repeatedly, because you have to select multiple times and add those areas up to get the total.  Is there a way to make it so you only have to select once to obtain the area to the right of the 9 kb band?

Thanks,

Dan




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