Plot Profile Question

Posted by Michael Lemieux on May 04, 2015; 7:46pm
URL: http://imagej.273.s1.nabble.com/Plot-Profile-Question-tp5012712.html

Hello ImageJ Community,

I have a question regarding the Plot Profile tool and how it generates
values for fluorescence intensity.  When a line selection of more than 1
pixel in width is drawn through a confocal image containing something
fluorescent, ImageJ should create a dataset where the "x" values represent
the length of the line and the "y" values represent the average intensity
of the pixels.  This works very nicely for a line that is drawn
horizontally.  If that same line is drawn vertically or diagonally,
however, the values that end up in the "y" column are not a simple average
of the pixels at any given position on that line.  So in summary, I am not
sure how ImageJ calculates those values for lines that are not perfectly
horizontal.  Any assistance would be greatly appreciated.  Thank you very
much.

Michael

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