http://imagej.273.s1.nabble.com/Plot-Profile-Question-tp5012712p5012714.html
> Hello ImageJ Community,
>
> I have a question regarding the Plot Profile tool and how it generates
> values for fluorescence intensity. When a line selection of more than 1
> pixel in width is drawn through a confocal image containing something
> fluorescent, ImageJ should create a dataset where the "x" values represent
> the length of the line and the "y" values represent the average intensity
> of the pixels. This works very nicely for a line that is drawn
> horizontally. If that same line is drawn vertically or diagonally,
> however, the values that end up in the "y" column are not a simple average
> of the pixels at any given position on that line. So in summary, I am not
> sure how ImageJ calculates those values for lines that are not perfectly
> horizontal. Any assistance would be greatly appreciated. Thank you very
> much.
>
> Michael
>
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