Posted by
Michael Schmid on
May 08, 2015; 8:47am
URL: http://imagej.273.s1.nabble.com/Changes-not-saved-in-tiff-image-tp5012767p5012773.html
Hi Malgosia,
the Brightness/Contrast settings don't modify the image contents, just the way how the image is displayed. For 8-bit and RGB images, you can click "Apply" in the B&C panel to make the change permanent.
There is no such option for 16-bit or floating-point (32-bit) images. If you need the image for display purposes only (not for further analysis), you can adjust B&C and then convert it to 8 bits (make sure that "scale when converting" is on in Edit>Options>Conversions), then save it as 8-bit image (PNG or whatever format you like).
If you want to keep the 16-bit data inside the display range, but truncate everything outside, you can use Process>Math>Min and Max to set all pixels outside the Min...Max range to the limits. If you have the 16-bit range set to "Automatic" in Edit>Options>Appearance, the image will be opened with that range. Of course, this modifies the image data.
Hope this helps,
Michael
________________________________________________________________
On May 8, 2015, at 06:35, Malgosia wrote:
> Hello,
>
> I am trying to save some changes I made to a 16-bit tiff file in Image J. I
> open the file, modify the brightness/contrast and then re-save as a tiff.
> But the new tiff image does not have the changes I made. It works when I
> save as jpeg, but I don't want to lose the image data.
>
> Anyone have a solution to this problem?
>
> Thanks!
>
> Malgosia
>
>
>
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