Posted by
Anderson, Charles (DNR) on
May 28, 2015; 7:43pm
URL: http://imagej.273.s1.nabble.com/How-to-calculate-the-particle-area-during-dissolution-tp5012970p5012976.html
Dimitris
Have you tried working with greyscale morphology, thresholding, and subtracting? See whether this helps you get started.
Charles
open("D:\\Documents\\Pictures\\PartBubbles.tif"); // change path and filename
//setTool("rectangle");
makeRectangle(389, 294, 233, 207);
run("Crop");
run("Duplicate...", "title=open5");
run("Gray Morphology", "radius=5 type=circle operator=open");
setAutoThreshold("Default dark");
//run("Threshold...");
//setThreshold(98, 255);
run("Convert to Mask");
run("Invert");
imageCalculator("Subtract create", "PartBubbles.tif","open5");
selectWindow("Result of PartBubbles.tif");
rename("particle.tif");
imageCalculator("Subtract create", "PartBubbles.tif","particle.tif");
selectWindow("Result of PartBubbles.tif");
rename("BubblesRemoved.tif");
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of d karampalis
Sent: Thursday, May 28, 2015 8:23 AM
To:
[hidden email]
Subject: How to calculate the particle area during dissolution?
Hello all,
I attached a couple of typical pictures of the dissolution of particles under the microscope.As you can see bubbles are around the particle so it make it very difficult to separate them.What i basically want to do it to isolate the information of bubbles and particle as a function of time.I tried to use some commands of ImageJ but its a way far more complicated.Could you please anyone help me with that.
Thanks in advance,
Dimitris
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