Explant migration assays

Posted by TimFeinstein on Jun 01, 2015; 8:05pm
URL: http://imagej.273.s1.nabble.com/Explant-migration-assays-tp5013005.html

Hello,

I have an assay that seems simple enough to describe, but I cannot find the ImageJ/FIJI tool that quite does what we need.  We have tissue explants transferred to a thin collagen gel, and we are measuring migration of cells in um from the edge of the explant.  We would automatically detect these nuclei and use, e.g. CellProfiler, but the throughput is not very high and manually marking cells should be faster than troubleshooting a segmenter.

This seems like it could be addressed with FISH tools - treat the explant as a "nucleus" or simply as a drawn oval and manually mark the nucleus of each migrating cell; the tool would measure the distance of each marked nucleus from the nearest edge of the explant.  Can anyone recommend a tool for this job?

Many thanks,


Tim

Timothy Feinstein, Ph.D.
Research Scientist
University of Pittsburgh Department of Developmental Biology


--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html