http://imagej.273.s1.nabble.com/Explant-migration-assays-tp5013005p5013022.html
probably the easiest way to have the same overlay for all stack slices is Image>Overlay>Add Image.
Also the stack can have a LUT. To create e.g. a 'red only' lut, you can use the LUT editor.
> I have an application where I need to overlay a single monochrome image (rendered, say, in Blue), over a monochrome STACK (rendered, say, in Green). All images are the same size in X and Y. I would like to scroll through the stack, and optionally have the ability to modify the blending of the Green and Blue (even simply on/off for the single image would be useful).
>
> Is this straightforward? Does anyone have a code snippet to share?
>
> Just to make it interesting, I *might* want to annotate the single image (what’s left? - in Red) - but this is not yet absolutely necessary, and I can do without it if it’s difficult.
>
> --
> Kenneth Sloan
>
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> Vision is the art of seeing what is invisible to others.
>
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>
>> On Jun 1, 2015, at 15:08 , Feinstein, Timothy N <
[hidden email]> wrote:
>>
>> Hello,
>>
>> I have an assay that seems simple enough to describe, but I cannot find the ImageJ/FIJI tool that quite does what we need. We have tissue explants transferred to a thin collagen gel, and we are measuring migration of cells in um from the edge of the explant. We would automatically detect these nuclei and use, e.g. CellProfiler, but the throughput is not very high and manually marking cells should be faster than troubleshooting a segmenter.
>>
>> This seems like it could be addressed with FISH tools - treat the explant as a "nucleus" or simply as a drawn oval and manually mark the nucleus of each migrating cell; the tool would measure the distance of each marked nucleus from the nearest edge of the explant. Can anyone recommend a tool for this job?
>>
>> Many thanks,
>>
>>
>> Tim
>>
>> Timothy Feinstein, Ph.D.
>> Research Scientist
>> University of Pittsburgh Department of Developmental Biology
>>
>>
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