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how to maintain 4 channels when using RGB color function

Posted by Vanessa Punal-2 on Jun 14, 2015; 8:51pm
URL: http://imagej.273.s1.nabble.com/how-to-maintain-4-channels-when-using-RGB-color-function-tp5013159.html

Hi ImageJ Community,


I am hoping someone can help me out with an issue I have encountered. I have taken several z-stack images which contains 4 channels (blue, green, red, and far red) and have maximum projected the data. Because I am stitching these images together, I need to convert each 4 channel maximum projection image into an RGB image. However, when I do this and then revert the image back to a composite, I always loose the 4th channel (in reality, the 4th channel becomes merged with the other 3 channels). How can I fix this? Thank you in advance!


Best,

Vanessa


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