Re: beads for Multiview Registration

Posted by Stephan Janosch on Jun 15, 2015; 10:50am
URL: http://imagej.273.s1.nabble.com/beads-for-Multiview-Registration-tp5013158p5013164.html

Hey Mario,


we use
http://www.emdmillipore.com/US/en/product/Fluorescent-Functionalized-Microspheres---F1-Z-030,MM_NF-39420083?bd=1

with a final concentration of 0.0005 %

And for imaging L1 c.elegans we usually go to full zoom with the LZ 1. I
hope this helps.

Working with 0.0004 % concentration resulted in difficult registration
situation.

Hope this helps and mybe Stephan Preibisch can put his concentrations
for from his early worm imaging.
Stephan

ps. Christopher Schmied might also have valuable infos fro you.

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Stephan Janosch
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https://transgeneome.mpi-cbg.de

Max Planck Institute of Molecular Cell Biology and Genetics
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