Posted by
Douglas Richardson on
Jun 16, 2015; 1:40am
URL: http://imagej.273.s1.nabble.com/beads-for-Multiview-Registration-tp5013158p5013176.html
We've tested a number of different sizes. We find 200nm beads work best
with our 20x/1.0 objective (we order fluorospheres from Life Technology and
use at a dilution of 1:500 000), but we need to go to 1um beads with our
5x/0.16 objective. These have to be used at a higher concentration, usually
1:2000 dilution from stock.
Personally I prefer 100nm beads for use with the 20x (as these are usually
too dim to be seen in the final reconstruction), but the processing
requires an extra step. Before registration, I adjust the min/max values
to completely saturate the sample (becomes a giant blob) and allow easy
visualization/registration of the beads). I convert this to 8bit and
perform the registration. I then use these registration files on the
original data.
Hope this helps!
-Doug
On Mon, Jun 15, 2015 at 6:53 AM, Stephan Janosch <
[hidden email]> wrote:
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