image summing details

Posted by Martin Ward-2 on Jun 22, 2015; 9:15am
URL: http://imagej.273.s1.nabble.com/image-summing-details-tp5013231.html

Hi,

I wish to take a series of consecutive images and merge them to obtain  
a mean grayscale value.  So far I have been creating a stack and then  
summing images from the Z-functions menu.  Finally I would select the  
SUM image and obtain the mean gray value.

However, I am not too sure what is happening 'behind the scenes' with  
this process. it does appear that there is some sort of autoscale  
process happening - would I be correct in thinking that this is the  
case?  I have had a look online but have not yet found and  
documentation or details of the image processing for any of the  
z-function.

Ideally I would like to avoid any autoscaling or be able to apply a  
fixed autoscale to different stacks in order to provide a realistic  
relative difference between different stacks.


Regards,
Martin.

--
The University of Edinburgh is a charitable body, registered in
Scotland, with registration number SC005336.

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html