Re: image summing details

Posted by Herbie on Jun 22, 2015; 10:22am
URL: http://imagej.273.s1.nabble.com/image-summing-details-tp5013231p5013232.html

Dear Martin,

could you please be more specific with what you are doing and what you
are suspecting?

E.g., I don't see a "Z-functions menu" but a "ZProjection"-dialog window
that is accessible from the "Image > Stacks > Z Project..."-submenu.
This dialog provides various "Projection type"-options. None of them is
called "SUM" but there is a corresponding option called "Sum Slices".

Do you speak of different operations?

Somehow puzzled

Herbie

:::::::::::::::::::::::::::::::::::::::::
Am 22.06.15 um 11:16 schrieb Martin Ward:

> Hi,
>
> I wish to take a series of consecutive images and merge them to obtain a
> mean grayscale value.  So far I have been creating a stack and then
> summing images from the Z-functions menu.  Finally I would select the
> SUM image and obtain the mean gray value.
>
> However, I am not too sure what is happening 'behind the scenes' with
> this process. it does appear that there is some sort of autoscale
> process happening - would I be correct in thinking that this is the
> case?  I have had a look online but have not yet found and documentation
> or details of the image processing for any of the z-function.
>
> Ideally I would like to avoid any autoscaling or be able to apply a
> fixed autoscale to different stacks in order to provide a realistic
> relative difference between different stacks.
>
>
> Regards,
> Martin.
>

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