Re: image summing details

Posted by Herbie on Jun 22, 2015; 11:11am
URL: http://imagej.273.s1.nabble.com/image-summing-details-tp5013231p5013233.html

Martin,

how do you expect the sum of several 8bit images to fit into the dynamic
range (0...255) of the resulting 8bit image?

Does this help?

The different appearance of objects in single slices after summing may
be explained by taking into account the properties of your stack images
and the 32bit result image.

HTH

Herbie

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Am 22.06.15 um 12:38 schrieb Martin Ward:

> Hi Herbie,
>
> Apologies for my confusing post, I was recalling the menu options from
> memory at the time.  Yes, you are correct I was using the sum slices command
> from the projection type menu.
>
> Upon summing the slices in my stack I notice that some objects that appear
> bright in a single frame are dulled following the stack slices being summed.
> I am assuming that this can only be the case if there is some sort of
> averaging/scaling occurring too?
>
> I have just noticed that my starting stack is comprised of 8-bit images and
> the summed image is 32-bit - should this cause any problems regarding the
> mean gray value that I would measure?
>
>
> Thanks,
> Martin

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