Find ROIs with maximal intensity increase in a time series

Posted by Bertrecht on Jun 25, 2015; 10:55am
URL: http://imagej.273.s1.nabble.com/Find-ROIs-with-maximal-intensity-increase-in-a-time-series-tp5013282.html

Hello everybody,

in one of my experiments I obtain a time series of one frame, which can be opened with imagej as a stack. I would like to find out, which ROIs within this frame show the strongest increase in intensity. For example, I want to divide the image into small rectangular ROIs and then compare each mean intensity ratio from the end frame to the first frame. Ideally, the macro will give me a normalized colour-coded intensity map on the basis of the ratios. Maybe there are already macros available for this purpose or you can help me by creating such macro, since I'm not very experienced with imagej until now.

Best

Bertrecht