Re: Find ROIs with maximal intensity increase in a time series

Posted by fabrice senger-2 on Jun 25, 2015; 11:35am
URL: http://imagej.273.s1.nabble.com/Find-ROIs-with-maximal-intensity-increase-in-a-time-series-tp5013282p5013283.html

Hi,

I guess if you do a Z-projection and go for standard deviation the siganl
should be the highest were the intensity increases the most.

Fabrice.

2015-06-25 12:55 GMT+02:00 Bertrecht <[hidden email]>:

> Hello everybody,
>
> in one of my experiments I obtain a time series of one frame, which can be
> opened with imagej as a stack. I would like to find out, which ROIs within
> this frame show the strongest increase in intensity. For example, I want to
> divide the image into small rectangular ROIs and then compare each mean
> intensity ratio from the end frame to the first frame. Ideally, the macro
> will give me a normalized colour-coded intensity map on the basis of the
> ratios. Maybe there are already macros available for this purpose or you
> can
> help me by creating such macro, since I'm not very experienced with imagej
> until now.
>
> Best
>
> Bertrecht
>
>
>
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