Re: Find ROIs with maximal intensity increase in a time series

Posted by Michael Schmid on Jun 25, 2015; 1:08pm
URL: http://imagej.273.s1.nabble.com/Find-ROIs-with-maximal-intensity-increase-in-a-time-series-tp5013282p5013291.html

Hi Bertrecht,

what about the following idea:
- Duplicate the first and last slice
- Subtract or divide these two images, with floating-point(=32-bit) output (Image>Process>Image Calculator)

You can also create a mask of your ROIs and then run the 'Particle Properties to Image' macro to replace the particles with their mean intensity. Thereafter subtract or divide.
  http://imagejdocu.tudor.lu/doku.php?id=macro:particle_properties_to_image

Michael
________________________________________________________________
On Jun 25, 2015, at 12:55, Bertrecht wrote:

> Hello everybody,
>
> in one of my experiments I obtain a time series of one frame, which can be
> opened with imagej as a stack. I would like to find out, which ROIs within
> this frame show the strongest increase in intensity. For example, I want to
> divide the image into small rectangular ROIs and then compare each mean
> intensity ratio from the end frame to the first frame. Ideally, the macro
> will give me a normalized colour-coded intensity map on the basis of the
> ratios. Maybe there are already macros available for this purpose or you can
> help me by creating such macro, since I'm not very experienced with imagej
> until now.
>
> Best
>
> Bertrecht

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