Activating different channels at different points in a 3-D projection?

Posted by zwein44 on Jul 01, 2015; 9:07pm
URL: http://imagej.273.s1.nabble.com/Activating-different-channels-at-different-points-in-a-3-D-projection-tp5013387.html

Hi everyone,

So I'm very new to imagej/ fiji and am currently attempting to make a 3-D "movie" of sorts as I rotate around two neurons. We imaged them with a confocal microscope and used three different signals: DAPI, MCM2, and Nestin. I am able to make a composite image, shave off unnecessary slices along the stack, and turn it into a 3-D projection that can rotate our view of the image by 360 degrees.

The issue I'm having however, is that I can seem to find a way to have certain channels "pop in" at different times. In short, I want to organize it so that first the few frames of the projection, we only see DAPI. At a different angle, I want to see both DAPI and MCM2, and then towards the end, I want to see all 3 channels. Is there a way to do this within the native fiji program or do I need a plugin?

Thanks!

-Zach