Sizing hollow, touching particles

Posted by lasercowboy on Jul 05, 2015; 6:39am
URL: http://imagej.273.s1.nabble.com/Sizing-hollow-touching-particles-tp5013424.html

Hi all,

I'm having a problem trying to size droplets in microscope images using (what I can ascertain from t'internet appear to be) standard methods within ImageJ.

I've tried various combinations of binarizing, thresholding, edge finding, etc. but I can't find something that works for all of the droplets within an image. I suffer from the dual problems of the droplets appearing "hollow" when binarizing/thresholding (and fill holes doesn't seem to like them) and in many cases droplets touching.

A typical image is shown at the following link:

https://lh3.googleusercontent.com/G8YVgXf2vj4YKhkOVu-ATD91DBsB7HzmkjfFUgRyVkc=w1280-h960-no

I feel like this should be fairly simple, but I'm not making headway. Hence asking the experts!

Any advice would be very gratefully received.

many thanks,
Rico