http://imagej.273.s1.nabble.com/what-s-the-best-way-to-process-3D-image-data-in-ImageJ-tp5013349p5013441.html
Thanks Michael.
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> On 6 Jul 2015, at 11:24, Doube, Michael <
[hidden email]> wrote:
>
> Dear Shameem,
>
>> On 30/06/15 12:54, Shameem Sampath wrote:
>> Hi List,
>> Coincidentally, I have a related query regarding analysing CAT scan Dicom images of knees in ImageJ.
>> Is there a way to measure trabecular orientation in the xyz planes?
>
> Trabecular orientation is usually measured using the mean intercept length (MIL) method, although in greyscale images, Fourier-based (autocorrelation) methods can work as well.
>
> The bigger problem you have is that if you have clinical CT images, you have relatively low resolution in the axial (z) direction compared to the in-plane resolution of the slices (xy) and trabecular size (in humans, no greater than 500µm diameter). Typical pixel spacings are something like 100-500 µm in xy and 500-2000µm in z. True resolution is usually somewhat less than that. Usually, z slice-spacing is 10-20× greater than xy pixel spacing. So that means it's very difficult to resolve the z element of any 3D trabecular orientation vector.
>
> If you really need trabecular orientation, you must have isotropic pixel spacing (same in x, y and z), otherwise your measurement is likely to be confounded by its interaction with pixel spacing.
>
> Best regards,
>
> Michael
>
>> Regards,
>> Shameem Sampath
>> The Bluespot Knee Clinic
>
> <
http://www.rvc.ac.uk>
>
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