Posted by
spt on
Jul 26, 2015; 12:21am
URL: http://imagej.273.s1.nabble.com/particle-analysis-within-region-of-interest-tp5013741p5013744.html
Thanks Jens, that does work and I imagine I could write a macro to do several ROIs at a time, but it puzzles me that it would require the duplication b/c the manual implies otherwise. So, I'm still thinking that I may be missing something fundamental. Very helpful nonetheless.
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of jens rietdorf
Sent: Saturday, July 25, 2015 7:01 PM
To:
[hidden email]
Subject: Re: particle analysis within region of interest
Dear Susan,
could it be a simple as using the command 'duplicate' after ROI selection and applying thus the thresholded measurement only at the copied subregion of the image?
Good luck, Jens
Visiting Scientist
Fundação Oswaldo Cruz - Ministério da Saúde, Centro de Desenvolvimento Tecnológico em Saúde (CDTS) Am 25.07.2015 16:23 schrieb "Travers, Susan" <
[hidden email]>:
> This should be simple but somehow I can't get it to work. I want to
> count particles in a specific region of interest. I have:
> Thresholded the image (using, threshold or color threshold-the
> particle analysis works correctly on the thresholded image) But when I
> try to count particles in a subregion, either by using the ROI manager
> or "selection" "specify", I still get the count that corresponds to
> ALL the particles in the image.
> At the same time, if I use the ROI manager and then the "measure"
> command, the measurements correspond to the ROI's that I've defined.
> Does anyone have a suggestion? Thanks so much.
>
> Susan
>
> Susan Travers
> Professor
> Division of Biosciences
> College of Dentistry
> The Ohio State University
>
> 305 West 12th Avenue
> Columbus, Ohio 43210
>
> 614-292-6366 (office)
> 614-457-6945 (fax)
> 614-361-0800 (cell)
>
>
> --
> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html>
--
ImageJ mailing list:
http://imagej.nih.gov/ij/list.html--
ImageJ mailing list:
http://imagej.nih.gov/ij/list.html