Re: particle analysis within region of interest

Posted by Peterbauer Thomas on Jul 26, 2015; 6:43pm
URL: http://imagej.273.s1.nabble.com/particle-analysis-within-region-of-interest-tp5013741p5013746.html

On 2015-07-26 20:18, Travers, Susan wrote:
> Dear Avital,
> Thanks for the tip. It should work, but the problem is that even when I select an ROI, the threshold is applied to the entire image.  This is despite the fact that the ROI is effective for other operations like adjusting brightness and contrast.  And the solution that was proposed by Jens yesterday (ie duplicating an ROI then thresholding) works, but only if the ROI is a rectangular shape... so, I still don't have a solution and I'm suspicious that a default is set up wrong somewhere in my program.

Rather than duplicating the image, you can copy selections of arbitrary
shape into a new image (either black or white, depending on the
background of your objects), and do the thresholding and particle
analysis there.

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