processing of noisy images with particles in bad contrast

Posted by M S on Jul 31, 2015; 12:30pm
URL: http://imagej.273.s1.nabble.com/processing-of-noisy-images-with-particles-in-bad-contrast-tp5013824.html

Good day, dear ImageJ users!
My field of work is totally not related to image processing, but therealways is a first time when you need to do something new. Having no idea how toproceed makes it difficult though.
I have acquired many images similar to what can be seen in particles.tif. Ifyou use your imagination then you can see approximately 58 black blobs which Ihave marked in markers_particles.tif. The problem is that very low exposition wasused (since the particles move really fast) and that creates all the noisewhere actual particles are drowning.
I have played with ImageJ/Fiji settings – Image arithmetics, Threshold,Enhance Contrast, Noise removal etc. in different combinations, but nothingbrings any satisfactory results. By satisfactory I mean more or less clearly visibleparticles that could be traced. If from these 50-something particles that canbe seen even half could be traced, that would be a great achievement. I haveattached a short preview of image sequence as well.
So the question – is it possible to automatically process these imagesequences in not time consuming way (manual processing of single image is notwhere I would like to spend my time) so final result would be a clear view ofat least some particles which could be traced using for example Mosaic’sParticle Tracker?
Sincerely,John

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