Re: run("Set Mearsurements...... How to pass a variable

Posted by tisalon on Aug 02, 2015; 5:39pm
URL: http://imagej.273.s1.nabble.com/run-Set-Mearsurements-How-to-pass-a-variable-tp3694651p5013851.html


Dear all I'm trying to write a macro that open all the movies (*.dv) inside a
file and then substract the background of two channels. This is the code
that I have till now but it seems that the command save does not work and
also does not make any substraction, just open the movies and split the
channels:

dir1 = getDirectory("C:\Users\p250572\Desktop\Santi\training");
list = getFileList(dir1);
dir2 = dir1+"analysed"+File.separator;
File.makeDirectory(dir2);
for (i=0; i<list.length; i++)
       {

       if (File.isDirectory(dir1+list[i])){}
       else{

               path = dir1+list[i];
               if (endsWith(path, ".db")){}
               else{

                       open(path);
                       if (bitDepth!=24){}  
                       else {
                               setBatchMode(true);
                               title = File.nameWithoutExtension ;
                               run("Split Channels");
                               close();
                                                                selectWindow(title);
                                                                run("Subtract Background...", "rolling=50");
                               saveAs("Tiff", dir2+title+"d0.tif");
                               saveAs("Tiff", dir2+title+"d1.tif");
                               close();
                               saveAs("Tiff", dir2+title+"d2.tif");
                               close();
                               setBatchMode(false);

                               }
                       }
               }
       }

Any piece of advice will be appreciate!

Best wishes;