Detecting cell spheroid on image with analyze particles

Posted by mikael.f on Aug 03, 2015; 12:27pm
URL: http://imagej.273.s1.nabble.com/Detecting-cell-spheroid-on-image-with-analyze-particles-tp5013863.html

Hello,

I wish to detect a cell spheroid in a picture taken by standard light microscopy. Despite having a proper threshold which locates the spheroid, the command "analyze particles" always detects objects from the opposite colour. For example, if I apply a threshold which "stains" the spheroid white, the command "analyze particles" detects dark regions. Now if the threshold "stains" the spheroid in black, the same option will detect objects in white. Is there a way to reverse this effect by modifying the "analyze particles" command?

*The picture is an RGB image which is converted to binary after thresholding.

Thank you,

Mikael F.