Hough transform question

Posted by David Gene Morgan on Aug 03, 2015; 4:02pm
URL: http://imagej.273.s1.nabble.com/Hough-transform-question-tp5013875.html

Hi,

        We have a series of electron microscope images of spherical viruses
that have been treated in different ways.  The images of the untreated
virus show (potentially overlapping) circular objects (dark against a
light background).  We suspect that various treatments are causing a
structural deformation and an initial question that we would like
answered is whether the treatments are affecting how "circular" the
virus particles are.

        I know that Hough transforms can be used to identify circular objects
and I suspect that approach could be used to identify the individual
virus particles in these images.  What I'd like to know is whether there
is then some way to quantify the "circularity" of them.

        The visible deviations from circularity can be as pronounced as
irregular shapes or as subtle as a transformation into slightly oval
shapes.  The treatments can also cause an increase in "broken" virus
particles (incomplete circles, for example).  We would like to be able
to quantify these effects.

        Any advice would be welcome.

--
                  David Gene Morgan
              Electron Microscopy Center
                   047D Simon Hall
                   IU Bloomington
                812 856 1457 (office)
                812 856 3221 (3200)
             http://bio.indiana.edu/~cryo

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