Analysis of Ca2+ waves dynamics with ImageJ

Posted by Olga_K on Aug 05, 2015; 2:03pm
URL: http://imagej.273.s1.nabble.com/Analysis-of-Ca2-waves-dynamics-with-ImageJ-tp5013907.html

Hello everyone,
I have an image time series from an LSM800 confocal microscope in .czi format. I image Ca2+ signal in cells with long processes. My task is to see the direction and the velocity of the Ca2+ wave movement over the distance of the cell process over time. Is there some way ImageJ can help me to do it? So far I only know how to import a .czi file in ImageJ and see the mean fluorescence in a ROI over time, but no idea how to track the movement of the fluorescent signal.
Thank you in advance, Olfa