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Re: Analysis of Ca2+ waves dynamics with ImageJ

Posted by Olga_K on Aug 07, 2015; 11:32am
URL: http://imagej.273.s1.nabble.com/Analysis-of-Ca2-waves-dynamics-with-ImageJ-tp5013907p5013947.html

Hi Pedro,
Thank you so much for your help! The first method is very easy to understand. I also tried to do the kymograph, but I have some concerns. I do not have only one wave travelling along the process during the recording, it looks there are 2-3 waves per recording time (see attached), in the kymograph won't they just overlay on each other? How can I be sure some fluorescence point in space and time still reflects the propagation of the first wave and not initiation of the second wave?