Posted by
Kischell, Eric R. on
Aug 07, 2015; 1:31pm
URL: http://imagej.273.s1.nabble.com/Hough-transform-question-tp5013875p5013948.html
David,
Could you please provide a link to sample images?
Thx e.-
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Michael Sarahan
Sent: Monday, August 03, 2015 1:06 PM
To:
[hidden email]
Subject: Re: Hough transform question
Hi David,
Have you thought of using image moments for the circularity? If they are distorted, the ratio of the principal axes might be a good measure. If your background is pretty uniform, this might work. Otherwise, the background might influence things too much.
Hope this helps...
Michael
On Mon, Aug 3, 2015 at 9:08 AM David Gene Morgan <
[hidden email]>
wrote:
> Hi,
>
> We have a series of electron microscope images of spherical
> viruses that have been treated in different ways. The images of the
> untreated virus show (potentially overlapping) circular objects (dark
> against a light background). We suspect that various treatments are
> causing a structural deformation and an initial question that we would
> like answered is whether the treatments are affecting how "circular"
> the virus particles are.
>
> I know that Hough transforms can be used to identify circular
> objects and I suspect that approach could be used to identify the
> individual virus particles in these images. What I'd like to know is
> whether there is then some way to quantify the "circularity" of them.
>
> The visible deviations from circularity can be as pronounced
> as irregular shapes or as subtle as a transformation into slightly
> oval shapes. The treatments can also cause an increase in "broken"
> virus particles (incomplete circles, for example). We would like to
> be able to quantify these effects.
>
> Any advice would be welcome.
>
> --
> David Gene Morgan
> Electron Microscopy Center
> 047D Simon Hall
> IU Bloomington
> 812 856 1457 (office)
> 812 856 3221 (3200)
>
http://bio.indiana.edu/~cryo>
> --
> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html>
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