Re: reUse Grid stitching generated coords
Posted by
Majurski, Michael Paul (Fed) on
URL: http://imagej.273.s1.nabble.com/reUse-Grid-stitching-generated-coords-tp5014013p5014014.html
Simone,
I do not know how to force the "Grid/Collection" stitching plugin to use a specific set of positions to assemble a new channel. As far as I understand it, using the positions from file gives the stitching tool a starting point from which to optimize translations.
However, I can add a plug for the stitching plugin we developed which I know can use coordinates generated on a previous stitching run to assemble a stitched image from new images. This seems to follow your use case of using a single channel to register the images, and then assemble stitched images from the other channels using those exact image positions.
The stitching plugin:
https://github.com/NIST-ISG/MISTInstall Guide:
https://github.com/NIST-ISG/MIST/wiki/Install-GuideThe FAQ about stitching secondary channels.
https://github.com/NIST-ISG/MIST/wiki/FAQ#how-to-stitch-from-metadatahttps://github.com/NIST-ISG/MIST/wiki/FAQ#stitching-multiple-channelsThanks,
~The NIST ISG Team
~Michael Majurski
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of simone codeluppi
Sent: Thursday, August 13, 2015 10:58 AM
To:
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Subject: reUse Grid stitching generated coords
Hi
My goal is to stitch a multi-channels image using the coordinates or overlapping of the tiles generated in the stitching of a reference channel (ex. DAPI staining). I first run the plugin stitching the Reference channel (grid setting) saving the TileConfiguration or TileConfiguration.registered file. I then I re-run the plugin on a different channel using the "Position from file" setting from TileConfiguration or TileConfiguration.registered but the stitching result is slightly different. Is there a way to "force"
the plugin to place the tiles using the previously generated coord?
Any help will be greatly appreciated.
Thanks a lot!
Simone
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