Posted by
Niko Ehrenfeuchter on
URL: http://imagej.273.s1.nabble.com/reUse-Grid-stitching-generated-coords-tp5014013p5014022.html
Hi Simone,
simply switch off the "compute overlap" option when re-using the
coordinates from a previous run.
For more details, see here:
http://fiji.sc/Image_StitchingCheers
Niko
On 13.08.2015 16:57, simone codeluppi wrote:
> Hi
> My goal is to stitch a multi-channels image using the coordinates or
> overlapping of the tiles generated in the stitching of a reference channel
> (ex. DAPI staining). I first run the plugin stitching the Reference channel
> (grid setting) saving the TileConfiguration or TileConfiguration.registered
> file. I then I re-run the plugin on a different channel using the "Position
> from file" setting from TileConfiguration or TileConfiguration.registered
> but the stitching result is slightly different. Is there a way to "force"
> the plugin to place the tiles using the previously generated coord?
>
> Any help will be greatly appreciated.
> Thanks a lot!
>
> Simone
>
>
>
>
>
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Niko Ehrenfeuchter | Image Analysis Specialist | Biozentrum, University
of Basel | Klingelbergstr. 50/70 | CH-4056 Basel
Phone: +41 (61) 26 72673 |
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