Posted by
Romain Guiet on
Aug 21, 2015; 1:31pm
URL: http://imagej.273.s1.nabble.com/coloring-more-than-3-channels-tp5014098.html
Dear all,
I’m writing today to give you the result of our thoughts and furthermore to get your feedback about a Look Up Table (LUT) issue.
For years, we used the Red (RGB 255;0;0), Green (RGB 0;255;0), and Blue (RGB 0;0;255) as the standard LUTs for coloring our Black&White images, so that they can be merged together.
Thankfully, some of us highlighted an issue encountered by people with color blindness and proposed to use a combination of Cyan(RGB 0;255;255), Yellow (RGB 255;255;0) and Magenta (RGB 255;0;255).
We are now facing a new challenge due to the increasing number of simultaneous stainings and the microscope capabilites to distinguish them. So now, some of us would like to merge more than 3 colors in an image.
It’s quite difficult to use a combination of Red, Green, Blue, Cyan, Yellow, and Magenta because the last three are all dependant equally of two of the RGB colors.
And splitting the data into a combination of 3 colors images can lead to some confusion.
So, we thought about using a set of 6 maximaly separated colors in RGB space.
Please find below a link to an explanatory document (in pdf):
https://drive.switch.ch/public.php?service=files&t=ce68ab027aeaea29fc56e2d5b35b301dThe list of numbers used to make the LUT (in pdf):
https://drive.switch.ch/public.php?service=files&t=9e33b36c9011034809fa5adb412934b6(or xlsx):
https://drive.switch.ch/public.php?service=files&t=a57fc9f007b59255174c285ecce35e61and you can download our LUTs here :
https://drive.switch.ch/public.php?service=files&t=c6d90322997d7be66fc6abe3e49daeb7(and soon on our Update Site because we encounter some trouble today…)
We are looking forward to your inputs about this,
Cheers,
Romain
---------------------------------------------------------------
Dr. Romain Guiet
Bioimaging and Optics Platform (PT-BIOP)
Ecole Polytechnique Fédérale de Lausanne (EPFL)
Faculty of Life Sciences
Station 19, AI 0140
CH-1015 Lausanne
Phone: [+4121 69] 39629
http://biop.epfl.ch/---------------------------------------------------------------
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