Fwd: Analyze labeled vesicles per cell

Posted by Fco. Javier Diez Guerra on Aug 22, 2015; 10:47am
URL: http://imagej.273.s1.nabble.com/Fwd-Analyze-labeled-vesicles-per-cell-tp5014109.html

Hi,

We want to automate the generation of ROIs that fit into the area
occupied by individual cells cultured on coverslips (HeLa, MDCK, COS7,
etc). The purpose is to analyze the distribution of labeled vesicles
(number, size, etc.) in a cell to cell basis.

I will appreciate any suggestion regarding suitable plasma membrane
stains that can be used in fixed cells to outline their contours and
help with the initial thresholding and segmentaton.

In addition, I will be grateful to listen to your comments on the most
appropriate method (public macro or plugin) to automate this task. We
have tried the Mosaic Suite for ImageJ/Fiji and also Cell Profiler in
coverslips which were also counterstained with DAPI (to act as a seed),
with very limited success.

Thanks

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Fco. Javier Díez Guerra, PhD
Profesor Titular
Centro de Biología Molecular Severo Ochoa
C/ Nicolás Cabrera, 1
Universidad Autónoma
Ctra Colmenar Viejo Km 15
Cantoblanco, 28049 Madrid
SPAIN

phone:  [phone]
e-mail: [hidden email]

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