Photobleaching

Posted by jross34 on Sep 02, 2015; 8:30pm
URL: http://imagej.273.s1.nabble.com/Photobleaching-tp5014227.html

I know there are lots of posts out there about how to correct for photo bleaching but what I haven't seen yet is how to correct for photo bleaching when you have a true signal at some point.

I'm curious to know if there's a plugin/macro out there that will allow me to set the exponent for bleach correction for only a certain window but then apply it to the whole series.

For example, I may image for 100 frames before I evoke a signal change and then continue to image for another 100 frames. Obviously applying a bleach correction for the full 200 frames doesn't work because it will flatten my evoked signal. Instead I would like to set the correction parameters within the first 100 frames and then apply it to all 200 frames.

Anyone have any suggestions?