http://imagej.273.s1.nabble.com/particle-aggregate-length-measurement-problem-tp5014272p5014273.html
> Hi all,
>
>
>
> I have a particle aggregate analysis problem and I hope to find some help
> here among all those ImageJ experts (for visualization of what I need to
> realize pls look at the attached .png picture):
>
> I am investigating particle aggregates - or to be more precise: I am
> investigating how particle aggregates dispense when being exposed to a
> flow.
> Therefore I have an optical monitoring and I take screenshots at different
> times (e.g. t1 and t2). Then I take the raw pictures, and do the following
> ImageJ operations:
>
> run("8-bit");
>
> run("Auto Threshold", "method=Minimum");
>
> run("Convert to Mask");
>
> As a result I get images like the ones I marked in the attached example
> with
> (1). Now, after setting a global scale, I need to measure the maximum
> distance between the most peripheral black pixels. I do that by drawing a
> horizontal line between the relevant pixels (in the example I indicated
> this
> with a green line) (2). Finally I can measure the line length to get the
> exact distance of particle dispersion (3).
>
> Due to the fact that I have a lot of pictures I need to analyze, I need an
> automatic batch process solution providing the following:
>
> . Recognizing the relevant peripheral pixels
>
> . Drawing a horizontal line between the pixels
>
> . Measuring the line length
>
> . Giving readout as a txt file that I can use for further
> analysis/plotting etc.
>
>
>
> Is it possible to realize that? I hope someone is willing to help me and
> generally I want to say thank you for taking your time reading my problem.
>
> Best,
>
> Steve
>
>
>
>
>
>
>
>
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