Re: Photobleaching

Posted by meeks on Sep 09, 2015; 3:33pm
URL: http://imagej.273.s1.nabble.com/Photobleaching-tp5014227p5014288.html

Hi.
Maybe I'm completely off with this....but I had once a similar problem when I used a method called FRET-mediated Protection Against Photobleaching (FPAP; quenching of the excited fluorochrome via FRET). The background problem was (partially) solved by subtracting the previous frame from the next one of a stack. This way the photobleaching was constantly pretty much removed but a signal on a spot where FPAP occurred appeared after a while.

Greetz,

>m