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Re: Photobleaching

Posted by jerie on Sep 09, 2015; 4:57pm
URL: http://imagej.273.s1.nabble.com/Photobleaching-tp5014227p5014290.html

have a look at http://fiji.sc/Bleach_Correction.

you find the function in FijI ...image...adjust...bleach correction. Select a method,
set a ROI to a fluorescent region, outside the region that is later photoconverted and try.

Cheers, Jens
Jens Rietdorf Visiting Scientist Fundação Oswaldo Cruz - Ministério da Saúde, Centro de Desenvolvimento Tecnológico em Saúde (CDTS), Rio de Janeiro, Brasil.