RE: Photobleaching

Posted by jross34 on Sep 10, 2015; 3:31pm
URL: http://imagej.273.s1.nabble.com/Photobleaching-tp5014227p5014310.html

Thanks so much! I've only tried it on a single stack so far but it seems to be doing exactly what I need! I'll keep testing it throughout the day. Again, I really appreciate it!

Sincerely,

Jordan M. Ross

Graduate Student

University of Tennessee Health Science Center

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From: Kota Miura [via ImageJ] [ml-node+[hidden email]]
Sent: Thursday, September 10, 2015 7:24 AM
To: Ross, Jordan Marie
Subject: Re: Photobleaching

Hi,

I think that's a good feature, so I test added an option to set range for
fitting. Please test:

https://www.dropbox.com/s/baex4obsb1kqpeq/CorrectBleach_-2.0.3-SNAPSHOT.jar?dl=0

Just place this jar file instead of the one in your plugin folder already
(better keep the old version somewhere, of course). If you have comments,
please do so at

https://github.com/fiji/CorrectBleach/issues/2

Cheers,
Kota

On Wed, Sep 2, 2015 at 10:30 PM, jross34 <[hidden email]> wrote:

> I know there are lots of posts out there about how to correct for photo
> bleaching but *what I haven't seen yet is how to correct for photo
> bleaching
> when you have a true signal at some point*.
>
> I'm curious to know if there's a plugin/macro out there that will allow me
> to set the exponent for bleach correction for only a certain window but
> then
> apply it to the whole series.
>
> For example, I may image for 100 frames before I evoke a signal change and
> then continue to image for another 100 frames. Obviously applying a bleach
> correction for the full 200 frames doesn't work because it will flatten my
> evoked signal. Instead I would like to set the correction parameters within
> the first 100 frames and then apply it to all 200 frames.
>
> Anyone have any suggestions?
>
>
>
> --
> View this message in context:
> http://imagej.1557.x6.nabble.com/Photobleaching-tp5014227.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--

-------------------------------------------------------------
Dr. Kota Miura <[hidden email]>

Senior Image Analyst / NINS Research Administrator
European Molecular Biology Laboratory
Meyerhofstr. 1, 69117 Heidelberg, GERMANY
Tel [phone]
Mobile [phone]http://cmci.embl.de
++++++++++++++++++

Dr. Kota Miura  <[hidden email]>
Project Associate Professor
National Institutes of Natural Sciences (NINS), Japan
Bonn Office: Ahrstr. 58, 53175 Bonn, Germany
Tel: [phone]http://www.nins.jp/english/
-------------------------------------------------------------

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