RGB conversion for multi-channel images

Posted by LIM Soon Yew John (IMB) on Sep 14, 2015; 7:20am
URL: http://imagej.273.s1.nabble.com/Analyze-Particles-changes-the-results-settings-tp5013849p5014327.html

Dear All,

I had noticed that for FIJI (ImageJ 2.0.0-rc-38/1.50b), it ignores the display  mode and active channel when it convert a multi-channels (single time point) to RGB. Please see the example macro below:

run("Fluorescent Cells (400K)");
wait(500);
Stack.setDisplayMode("color");
Stack.setChannel(1);
wait(500);
run("RGB Color");

From the above macro, the final image from current version will always be a RGB composite image instead of RGB stack of different channels unlike previous version of ImageJ.

The current version works as normal for multi-channel images with multiple time-points / slices when I convert them to RGB. Only those with single time-point / slice failed to work as normal.

Best Regards,
John
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