Re: Calculate bubble volume

Posted by Rocco D'Antuono on
URL: http://imagej.273.s1.nabble.com/Calculate-bubble-volume-tp5014331p5014332.html

Hi Dimitri, I would do an approximation as follow:
-since you have not a z-stack (so you cannot segment the whole object), assume that the bubbles are quite round objects, without any large irregularity; this give me a sphere in 3D;
- measure Feret's diameter (see http://rsbweb.nih.gov/ij/docs/guide/146-30.html#toc-Subsection-30.7) and take a mean value between "Feret" and "MinFeret" measurements;
- from the diameter "d", calculate the volume V=(4*pi*(d/2)^3)/3.
Hope this would help,
best regards,
Rocco