Help measuring migration through neurospheres!
Posted by smrpre on Sep 17, 2015; 4:49pm
URL: http://imagej.273.s1.nabble.com/Help-measuring-migration-through-neurospheres-tp5014350.html
Hello all,
I'm having some issues figuring out an easier way to measure migration via my neurosphere assay. Essentially, for my assay, I form neurospheres by plating neural stem cells in absence of any coating substrate. Without coating, the neural stem cells free float in the media and over time aggregate to form spheres known as neurospheres. Once spheres are formed in the suspension, I collect and replate them onto dishes that are coated. The coating allows for the neurospheres to stick and for cells to migrate out from the original sphere. An example of what this looks like can be seen below.
In order to quantify migration, I currently measure inner area of the sphere (which is the bright center) and subtract it from total area of the sphere. My measurements are done by manually tracing both the inner and outer sphere in image j using the free hand tool. I trace around all the outer cells and essentially trace a circle around the inner mass However, as I acquire more spheres this is becoming very time consuming! Does anyone know of an easier way to do this????
URL: http://imagej.273.s1.nabble.com/Help-measuring-migration-through-neurospheres-tp5014350.html
Hello all,
I'm having some issues figuring out an easier way to measure migration via my neurosphere assay. Essentially, for my assay, I form neurospheres by plating neural stem cells in absence of any coating substrate. Without coating, the neural stem cells free float in the media and over time aggregate to form spheres known as neurospheres. Once spheres are formed in the suspension, I collect and replate them onto dishes that are coated. The coating allows for the neurospheres to stick and for cells to migrate out from the original sphere. An example of what this looks like can be seen below.
In order to quantify migration, I currently measure inner area of the sphere (which is the bright center) and subtract it from total area of the sphere. My measurements are done by manually tracing both the inner and outer sphere in image j using the free hand tool. I trace around all the outer cells and essentially trace a circle around the inner mass However, as I acquire more spheres this is becoming very time consuming! Does anyone know of an easier way to do this????
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