Posted by
daschneider9 on
URL: http://imagej.273.s1.nabble.com/Red-blood-cells-tp5014349p5014354.html
So by green do you mean trouble differentiating a fluorophore fluorescence in cells of interest from autofluorescence in RBCs? If so, then there are ways to reduce autofluorescence in RBCs that might help. If not, then more info needed.
Dave
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Petkov, Daniel
Sent: Thursday, September 17, 2015 11:21 AM
To:
[hidden email]
Subject: Re: Red blood cells
Yes but my macrophages in muscle are long and when tissue sections are prepared cells are cut unevenly and one cell may appear as rounded multiple objects (rbc) far from the nuclei.
Daniel
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Schneider, David A.
Sent: Thursday, September 17, 2015 1:10 PM
To:
[hidden email]
Subject: Re: Red blood cells
Only count cells that have a nucleus?
Dave
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Petkov, Daniel
Sent: Thursday, September 17, 2015 9:42 AM
To:
[hidden email]
Subject: Red blood cells
Hello,
I am counting cells stained in green with the Cell counter. Red blood cells produce very strong green signal. How can I eliminate (reduce) the likelihood that I will count rbc instead of my target cells?
Thank you, Daniel
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