Color colocalization of 2 channels (Z-stacks) in ImageJ

Posted by BrunoNP on Sep 21, 2015; 3:57am
URL: http://imagej.273.s1.nabble.com/Color-colocalization-of-2-channels-Z-stacks-in-ImageJ-tp5014383.html

Hello everyone,

I have two z-stacks ("2 channels") and I need to quantify the number of colocalization points between the two z-stacks. I've only found plugins allowing me to analyze the full projection images of the z-stacks but I need to find the colocalization points while considering the 3D nature of my images.

Do you know any plugin in ImageJ that would allow me to this in either a 3D visual representation or just by comparing each image of each z-stack consecutively?
I would like to be able to visualize the colocalization points in case any manual correction may be needed.

Thank you in advance